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KMID : 0370220150590030113
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Yakhak Hoeji
2015 Volume.59 No. 3 p.113 ~ p.119
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Anti-oxidant Activity of Phenolic Compounds and Simultaneous Contents Determin using HPLC-UV from the Akebiae caulis
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Kyung Min-Ji
Jeon Hye-Jin
Whang Wan-Kyunn
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Abstract
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Akebiae caulis have been used in folk medicines for diuretic, menstrual pain, and diuretic pain. It has been also resolved nephritis and cystitis. In this study, we isolated three phenolic compounds from 70% methanol extract using the open column chromatography. These isolated compounds which were 2-(3,4-dihydroxyphenyl)-ethyl-β-D-glucopyranoside, 5-O-caffeoylquinic acid, and syringoylglycerol 2-O-β-D-glucoside were determined by physico-chemical apparatus. Furthermore, we conducted DPPH and ABTS assay in order to screen the antioxidant activity of isolated three compounds. Also, we developed a rapidly HPLC-UV analysis method of two phenolic compounds (2-(3,4-dihydroxyphenyl)-ethyl-β-Dglucopyranoside, 5-O-caffeoylquinic acid) for evaluating the Akebiae Caulis collected 30 samples from different regions. From the experiments, all three isolated compounds showed the significant antioxidant activity. We suggested that the content criteria of marker compounds were shown by a simple and rapid HPLC-UV method. The contents respectively were 0.009% (2-(3,4-dihydroxyphenyl)-ethyl-β-D-glucopyranoside) and 0.036% (5-O-caffeoylquinic acid).
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KEYWORD
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Akebiae caulis, 2-(3,4-dihydroxyphenyl)-ethyl-β-D-glucopyranoside, 5-O-caffeoylquinic acid, syringoylglycerol 2-O-β-D-glucoside, antioxidant activity, HPLC-UV
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